Application of SRAP in the genetic diversity of Tricholoma matsutake in northeastern China

Abstract

Tricholoma matsutake is an ectomycorrhizal (ECM) fungus that produces economically important mushrooms. The study firstly applied SRAP technique into genetic diversity of T. matsutake. A total of 129 strains from 13 geographical locations in northeastern China, were amplified by using selected 12 primer pairs. The polymorphic band number amplified by each primer pair ranged from 7 to 13. In total 154 bands were observed, of which 118 were polymorphic (76.62%). Abundant genetic variation was detected within individual populations. Dongning maintained a higher genetic diversity while Hunchun was lower. The analyses found a significant positive correlation between genetic distance and geographical distance and no correlation between genetic distance and altitudinal differences among populations. Based on the UPGMA cluster diagram, the 13 populations may be divided with the genetic distance of 0.035 into three groups.

Key words: Genetic diversity, Tricholoma matsutake, fungi, sequence related amplified polymorphism (SRAP).