Assessment of genetic diversity in French bean (Phaseolus vulgaris L) based on RAPD marker

Abstract

RAPD molecular markers were used to evaluate the genetic diversity in the fourteen varieties of French bean (Phaseolus vulgaris) of three eco-geographical regions of Bangladesh. Out of the 20 primers only, 6 yielded polymorphic banding patterns. In total, 40 different DNA bands were reproducibly obtained, out of which 28 (70%) were polymorphic. The polymorphisms were scored and used in band-sharing analysis to identify genetic relationships. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the 14 genotypes into two major groups. Pair-wise variety comparison of the varieties showed that inter-variety similarity indices for PV004 versus PV005 (98.51) was higher than all other varietals pairs. On the other hand, inter-cultivar similarity index for BARI 01 versus PV009 (59.58) cultivar was lower than all other cultivar pairs. Therefore, the inherent simplicity and efficiency of RAPD analyses will be to facilitate the construction of RAPD-based genetic linkage maps in French bean.

Key words: RAPD, French bean, polymorphism.