Diversity of arbuscular mycorrhizal fungi in Camellia sinensis in Uttarakhand State, India

Abstract

Many microorganisms form symbioses with plants that range on a continuous scale, from parasitic to mutualistic. Among these, the most widespread mutualistic symbionts is the arbuscular mycorrhiza, formed between arbuscular mycorrhizal (AM) fungi and vascular flowering plants and other plants. A study of diversity of arbuscular mycorrhizal fungi in Camellia sinensis was conducted in four plantation territories of Uttarakhand. Microscopic analysis of the mycorrhizal status of roots has revealed that samples from all four locations belonged only to AM fungi. The mycorrhizal colonization level was found high thus reflecting the mycotrophic nature of C. sinensis (L.). Results of isolation and identification of spores from all field-collected soil samples has revealed relatively higher spore count. All recovered spores were found to belong to the Glomales Order, represented by Glomaceae family. Isolation has also brought into notice that three groups were dominant: the first one included light yellow colored spores, second, corresponded dark yellow-brown colored spores and third, magenta colored spores. The morphological characters indicated that the spore populations consisted of 3 – 6 morphotypes. The Glomus genus was represented by three species; that is, Glomus mossae, Glomus fasciculatum, Glomus sp. 1 (an unidentified species).

Keywords: Glomus mossae, Glomus fasciculatum, Mycorrhiza, Camillia sinensis

African Journal of Biotechnology Vol. 9(33), pp. 5313-5319, 16 August, 2010