Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Draga
cevka and cv. Jelica)

Abstract

An efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragaevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragaevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragaevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.