Influence of genotype and plant growth regulator on somatic embryogenesis in rapeseed (Brassica napus L.)

Abstract

Two genotypes of Brassica napus species (Talayeh and RGS003) and the explants segment (hypocotyls and cotyledon) were tested for their potential to produce somatic embryos in in vitro condition. The effect of genotype, different explants and also different concentrations of plant growth regulators (PGRs) including: -naphthalene acetic acid (NAA), 2,4-Dichlorophenoxy acetic acid (2,4-D), 6-benzyl amino purine (BAP), were investigated. The basal medium was Murashige and Skoog (MS)
supplemented with 2% sucrose and 1% agar. Explants plated on different callus induction medium and each 4 week was subculture in the same medium. The results showed that ‘Talayeh’, in terms of regeneration, had significant difference with RGS003 and produce more somatic embryos. In this genotype, modified MS medium containing 2% sucrose, 6 mg l-1 sodium chloride (NaCl), 3 mg l-1 BAP, 2 mg l-1 NAA and 2 mg l-1 2,4-D was the most efficient medium for somatic embryogenesis in ‘Talayeh’
and also, hypocotyl explants were found to be more suitable than cotyledon for somatic embryogenesis.