Effect of some metal-containing compounds and fertilizers on mycoparasite Trichoderma species mycelia growth response

Abstract

Response of three biocontrol agents including Trichoderma harzianum T969, Trichoderma hamatum T614 and Trichoderma virens T525 in the presence of 0, 50, 91.2, 166, 302, 550 and 1000 mg/l concentrations of MgSO4.7H2O, ZnSO4.7H2O, CuSO4, FeSO4.7H2O, MnSO4.H2O, CoCl2.6H2O, CaCl2.2H2O and (NH4)2SO4 via poisoned medium method, were studied. Radial growth of the Trichoderma isolates were recorded 48 and 72 h after inoculating. For each experimental set, experimental design used was completely randomized design (CRD) through factorial experiment with 3 factors. Also, inhibition percentage of the fungus mycelia growth due to metal containing compound at the inhibitory effect
exhibited compound was calculated. The experiments indicated that Trichoderma species mycelia response to the compound differed noticeably in their sensitivities to the metal containing compounds and fertilizers exposure. ZnSO4.7H2O, CuSO4, FeSO4.7H2O, MnSO4.H2O and CoCl2.6H2O compounds were effective as radial growth inhibitors, though (NH4)2SO4, MgSO4.7H2O and CaCl2.2H2O were found to be non-toxic to the Trichoderma species. Meanwhile, CuSO4 indicated the strongest inhibition towards the in vitro mycelia expansion of the fungi.