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Fish powder hulled grain of wheat heads and viscera alkaline protease Bacillus licheniformis NH1.
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The use of an economical medium for the production of alkaline serine proteases by Bacillus licheniformis NH1
Abstract
The present study is concerned with the selection of new economical media based on agricultural and marine-processing by-products for the production of alkaline proteases by Bacillus licheniformis NH1. Powders from different fish species were prepared and then tested as growth media at a concentration of 10 g/l for proteases production by NH1 strain. Powder prepared from whole Sardinelle was found to be the best substrate for the production of the alkaline protease. The NH1 strain exhibited a slightly
greater protease production (2927 U/ml) in medium containing only whole Sardinelle powder than that obtained in control medium (2800 U/ml). Proteases were produced even when strain NH1 was cultivated in medium containing only powder prepared from combined heads and viscera Sardinelle (CHVSP), about 2117 U/ml. Protease production was also carried out in media containing hulled grain of wheat, a by-product of semolina factories, as carbon source. Maximum activity (2517 U/ml) was achieved when the strain was grown in medium containing hulled grain of wheat (10 g/l), casein peptone (2 g/l), K2HPO4 (0.5 g/l) and KH2PO4 (0.5 g/l). Moreover, protease production was considerably enhanced when the
strain was grown in medium containing both hulled grain of wheat and CHVSP as carbon and nitrogen sources, respectively, (4771 U/ml). The study shows that hulled grain of wheat and powders from fishery by-products could be utilized as bacterial substrates for the production of alkaline proteases by B. licheniformis NH1.
greater protease production (2927 U/ml) in medium containing only whole Sardinelle powder than that obtained in control medium (2800 U/ml). Proteases were produced even when strain NH1 was cultivated in medium containing only powder prepared from combined heads and viscera Sardinelle (CHVSP), about 2117 U/ml. Protease production was also carried out in media containing hulled grain of wheat, a by-product of semolina factories, as carbon source. Maximum activity (2517 U/ml) was achieved when the strain was grown in medium containing hulled grain of wheat (10 g/l), casein peptone (2 g/l), K2HPO4 (0.5 g/l) and KH2PO4 (0.5 g/l). Moreover, protease production was considerably enhanced when the
strain was grown in medium containing both hulled grain of wheat and CHVSP as carbon and nitrogen sources, respectively, (4771 U/ml). The study shows that hulled grain of wheat and powders from fishery by-products could be utilized as bacterial substrates for the production of alkaline proteases by B. licheniformis NH1.
