Medium optimization for acarbose production by Actinoplanes sp. A56 using the response surface methodology

Abstract

In order to improve the production of acarbose, the fermentation medium of acarbose-producing strain Actinoplanes sp. A56 was optimized in this paper. Firstly, fractional factorial design was employed to investigate the influences of glucose, maltose, corn steep liquor, soy bean flour and monosodium glutamate on acarbose production, and the results revealed that maltose and corn steep liquor had significant and positive effects. Then, the two significant factors used the method of the steepest ascent to reach the yield plateau of acarbose. To explore the best condition obtained by the steepest
method, the concentrations of maltose and corn steep liquor were further optimized with central composite design and response surface analysis. As a result, the acarbose production was increased from 837 to 1043 mg/L by using this optimization strategy.