Study on serum fluorescence spectra based on wavelet transform

Abstract

Blood plays an important role in clinical diagnosis and treatment and as such, the analysis of blood spectrum will be of very important practical significance. Serum fluorescence emission intensity is closely related with the excitation wavelength; when the excitation wavelength is 230 nm, the blood lipid concentration and fluorescence intensity was significantly correlated. On the contrary, blood sugar was almost with no effect on the strength. Wavelet analysis was used in signal de-noising to get a wide range of applications. In this paper, fluorescence spectrum was divided into four layers by db4 wavelet, according to the principle of stein unbiased likelihood estimate.To choose the threshold, noise was removed and reconstruction signal received. This paper studied the correlation between blood lipid concentration and original fluorescence intensity, reconstruction fluorescence intensity and the fourth layer fluorescence strength. Some significant results were achieved, providing an experimental basis for further study on the fluorescence spectrum of blood.