Standardization of RAPD assay for genetic analysis of olive

Abstract

This study aimed at the standardization of PCR conditions for the purpose of determining the genetic similarities and distances in wild and cultivated olives collected from Izmir and Manisa provinces in Aegean Region. Three different PCR mix and three different PCR cycle as well as 20 primers from OP-I
series were tried in 13 wild and 12 cultivated samples. A suitable PCR protocol was developed and evaluable bands were derived for further studies. Required result could not be obtained from PCR Mixes I and II and also PCR cycles I and II; however, favorable results were attained by PCR Mix III and
PCR cycle III. Evaluable bands were obtained for defining the olive samples by using primers from OP-I. Thus, it was concluded that RAPD profiles are effective in the study of genetic similarities and distances of wild and cultivated olives.