Seed storage protein components are associated with curled cotyledon phenotype in soybean

Abstract

Local soybean cultivar “Nannong 94-16” was treated with NaN_3-⁶⁰Co ray and EMS separately, and two curled cotyledon mutants were obtained. Local soybean cultivar “TSB” was treated with ⁶⁰Co ray and one curled cotyledon mutant was obtained. Two-dimension electrophoresis analysis was employed to determine the differential proteins between three mutants and their wild types. The results showed that there were three common differential protein spots between the mutants and their wild types. Further analysis suggests that the two increased protein spots in mutants were both globulin subunit G3/A1aB1b, while the decreased protein spot in mutants was lectin. These results indicate that there
may be an association between protein components (glycinin subunits G3/A1aB1b, lectin) and the curled cotyledon phenotype in soybean.