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DOI:
10.5897/AJB09.1046Keywords:
Phytoplasma polymerase chain reaction cloning 16SrDNA banana phylogenetic analysis
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Molecular diagnostics and phylogenetic analysis of ‘Candidatus phytoplasma asteris’ (16SrI- Aster yellow group) infecting banana (Musa spp.)
Abstract
The presence of phytoplasma in diseased banana plants exhibiting acute stunting symptoms was detected by the polymerase chain reaction using a primer set specific for the 16SrRNA gene of phytoplasma. The amplified DNA fragments of 1500 bp were cloned in pGEMT-Easy plasmid cloning
vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the ‘Candidatus phytoplasma asteris’ (16Sr I-Aster yellow) group.
vector and sequenced. The BLASTN and phylogenetic analyses revealed the infecting agents to be the closely related members of the ‘Candidatus phytoplasma asteris’ (16Sr I-Aster yellow) group.
