Callus regeneration from stem explants of Pseudarthira viscidplanta (L.) Wight and Arn. – a vulnerable medicinal

Abstract

An

in vitro propagation protocol has been developed for Pseudarthira viscida (L.) Wight and Arn. (Papilionaideae), a vulnerable medicinal plant from stem callus was used for axillary shoot multiplication. The plant was standardized by using MS (Murashige and Skoog) medium containing 3% (w/v) sucrose, supplemented with a cytokinin (BAP, KN) maximum number of shoot (29.87±5.34) were observed on the medium containing 0.6 mg/l BAP after three weeks of culture. Single shoots were induced and elongated on MS medium supplemented with BAP+NAA (0.4 + 0.04 mg/l). The elongated shoots were rooted in half MS medium with 0.4 mg/l IBA, and then were successfully hardened and transferred to the field.