Cryopreservation of seeds of lily [Lilium Iedebourii (Baker) Bioss]: Use of sucrose and dehydration

Abstract

Cryopreservation of germplasm at liquid nitrogen (-196°C) is a perfect method for the long-term conservation of plant genetic resources. A cryopreservation process using dehydration was performed for seeds of lily [

Lilium Iedebourii (Baker) Bioss]. Seeds were subjected to a rapid freezing protocol in liquid nitrogen following dehydration and treatment with 0.75 M sucrose for 1 h. Survival after freezing was nil for control seeds and 75% for seeds treated with sucrose and dehydration.