Pheno- and genotyping of Pasteurella multocida isolated from goat in Iran

Abstract

In this study, phenotype, capsular type and some virulence factors (pfha1, tbpA, and toxA) of caprine

Pasteurella multocida were described in 10 isolates from goat. Two biochemical biovars were recognized among the isolates based on dulcitol and sorbitol fermentation. The isolates belonged to biovars P. multocida sub. multocida (80%) and P. multocida sub. septica (20%). Capsular typing of isolates by PCR demonstrated two capsular types A (90%) and D. Among 3 virulence genes detected by PCR, we found a remarkable high prevalence of tbpA (80%) and toxA (70%) genes among caprine isolates. The high prevalence of these genes owning association of disease status among healthy animals showed high potential of the strains in induction of disease. The high prevalence of toxA and tbpA among goat is very similar to the ones in sheep isolates. Comparison of virulence genes profile showed possibility of P. multocida transmission between sheep and goat.