Molecular characterization of 26S proteasome regulatory subunit in dermatophyte pathogen Trichophyton verrucosum

Abstract

Trichophyton verrucosum is a zoophilic dermatophyte, which causes dermatophytosis infection in human as well as animals. 26S proteasome is an important protein in eukaryotic cells that is involved
with degradation of unneeded or damaged proteins, when tagged with ubiquitin. In this study, we characterized the 26S Proteasome regulatory subunit gene in dermatophyte pathogen T. verrucosum.
High molecular weight DNA has been isolated from T. verrucosum and utilized with pairs of specific nucleotides primers, designed from highly preserved regions of the 26S proteasome regulatory subunit
genes. Obtained DNA fragments were sequenced and the results were analyzed in GenBank. This DNA fragment, which contains no intron within its open reading frame, encodes a polypeptide with 332 amino acids. The characterized PCR fragments revealed significant homology with other 26S
proteasome regulatory subunit genes in GenBank.