Antiproliferative heparin (glycosaminoglycans) isolated from giant clam (Tridacna maxima) and green mussel (Perna viridis)

Abstract

Heparin was isolated from two bivalve mollusks, Tridacna maxima (giant clam) and Perna viridis (green mussel). The isolated heparin was quantified in crude as well as purified samples and they were estimated as 2.72 and 2.2 g/kg (in crude) and 260 and 248 mg/g (in purified samples) in T. maxima and P. viridis, respectively. The antiproliferative activity of both the samples performed with pulmonary artery smooth muscle cells (PASMC) indicate a dose dependent manner. Among these two clams, P.
viridis heparin showed higher antiproliferative activity than that of T. maxima.