Platelet aggregation, antiglycation, cytotoxic, phytotoxic and antimicrobial activities of extracts of Nepeta juncea

Abstract

Various subsequent fractions of Nepeta juncea were subjected to test against platelet aggregation, antiglycation, cytoxicity, phytotoxicity and antimicrobial activities. Methanolic extract inhibited arachidonic acid (AA) induced platelet aggregation (IC50 35 ug/ml) and platelet activating factor (PAF)-induced platelet aggregation (IC50 38 ìg/ml) in a dose dependant fashion. In case of antiglycation bioassay, n-hexane fraction showed highest inhibitory activity (74.3%), followed by chloroform (72.4%) and water fractions (64.7%). The order of toxicity for brine shrimps was water > chloroform > n-hexane > N-butanol. Except n-hexane fraction (70% inhibition) at higher concentration (1000 ug/ml), n-butanol, chloroform and water fractions exhibited non-significant phytotoxic activity at even highest dose against
Lemna minor. No significant inhibitory effect of these fractions could be observed against these bacteria and fungi except, n-butanol fraction against Bacillus subtilis (27.0%), Shigella flexneri (30.7%) and
Staphylococcus aureus (42.3%). The chloroform fraction showed 25% antibacterial activity against Salmonella typhi only. Each fraction might have variable effects in different bioassays.