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Monoclonal antibodies polyclonal antibodies triple-antibody sandwich enzyme-linked immunosorbent assay dot-immunobinding assays.
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Development of rapid, specific and sensitive detection of Cucumber mosaic virus
Abstract
Cucumber mosaic virus (CMV) causes major losses to thousand agricultural and horticultural crops around the world. Unlike other plant pathogens, there are no direct methods available yet to control
viruses and, consequently, the current measures rely on indirect tactics to manage the viral diseases. Hence, methods for detection and identification of viruses, both in plants and vectors, play a critical
role in virus disease management. A rapid assay for diagnosis of CMV, which can be employed in both laboratory and field, is essential. Therefore, this study was undertaken to develop a procedure for
detection of the CMV in infected plants using a monoclonal and polyclonal antibodies. Dotimmunobinding assays (DIBA) are useful alternatives to microtitre plate enzyme-linked immunosorbent
assay (ELISA). Nine monoclonal antibodies were readily used for detected CMV by TAS-ELISA and DIBA of infected plants. DIBA has about the same sensitivity as ELISA in microtiter plates, but it has
the additional advantages of simplicity, quick completion in the field or office on large numbers of samples, economy, and can be quantified using densitometry.
viruses and, consequently, the current measures rely on indirect tactics to manage the viral diseases. Hence, methods for detection and identification of viruses, both in plants and vectors, play a critical
role in virus disease management. A rapid assay for diagnosis of CMV, which can be employed in both laboratory and field, is essential. Therefore, this study was undertaken to develop a procedure for
detection of the CMV in infected plants using a monoclonal and polyclonal antibodies. Dotimmunobinding assays (DIBA) are useful alternatives to microtitre plate enzyme-linked immunosorbent
assay (ELISA). Nine monoclonal antibodies were readily used for detected CMV by TAS-ELISA and DIBA of infected plants. DIBA has about the same sensitivity as ELISA in microtiter plates, but it has
the additional advantages of simplicity, quick completion in the field or office on large numbers of samples, economy, and can be quantified using densitometry.
