Article Sidebar
Article Details
Issue
Section
Articles
Copyright for articles published in this journal is retained by the journal.
Main Article Content
Determination of degradability of treated soybean meal and its proteins fractions
Abstract
This study was carried out to determine the effects of xylose and moisture heating treatments (0, 5 and 10 g/kg DM) of soybean meal (SBM) on in situ rumen degradability characteristics in cow and its
proteins sub-units fractions by a SDS-PAGE discontinues system. In the experiments, three ruminally cannulated male Taleshi cow were used to measure in situ rumen degradability characteristics of dry
matter (DM) and crude protein (CP) of different treated SBM samples. SBM were treated with xylose (5 and 10 g/kg DM) and moisture heating. Xylose and moisture heated treatment significantly (P<0.05)
reduced degradability values of DM and CP for SBM. Rumen effective protein degradability values of SBM (k = 0.02, 0.05 and 0.08 h-1) were significantly decreased by all treatments (P<0.05). Effective
degradability of DM (k = 0.05 h-1) were 65.17, 64.90, 61.77 and 58.50 g/kg and CP was 63.77, 61.97, 60.83 and 60.23 g/kg for NTSBM (non treated SBM), TSBM (SBM + 30 min moisture heating treated), TSBM 5 g/kg (SBM + 30 min moisture heating treated + 5 g/kg xylose) and TSBM 10 g/kg (SBM + 30 min moisture heating treated + 10 g/kg xylose) treatments, respectively. Treatment of SBM with 5 and 10 g/kg of DM caused a reduction in the dry matter and effective crude protein degradability compared with NTSBM. Electrophoretic patterns of untreated and treated SBM protein residues revealed that -conglycinin , and subunit were degraded completely within 4h of incubation in the rumen, whereas the acidic and basic subunits of glycinin were degraded after 48h incubation in treated SBM with Xylose. It is concluded that SBM proteins can be effectively protected from degradation in the rumen by xylose and moisture heating treatment.
proteins sub-units fractions by a SDS-PAGE discontinues system. In the experiments, three ruminally cannulated male Taleshi cow were used to measure in situ rumen degradability characteristics of dry
matter (DM) and crude protein (CP) of different treated SBM samples. SBM were treated with xylose (5 and 10 g/kg DM) and moisture heating. Xylose and moisture heated treatment significantly (P<0.05)
reduced degradability values of DM and CP for SBM. Rumen effective protein degradability values of SBM (k = 0.02, 0.05 and 0.08 h-1) were significantly decreased by all treatments (P<0.05). Effective
degradability of DM (k = 0.05 h-1) were 65.17, 64.90, 61.77 and 58.50 g/kg and CP was 63.77, 61.97, 60.83 and 60.23 g/kg for NTSBM (non treated SBM), TSBM (SBM + 30 min moisture heating treated), TSBM 5 g/kg (SBM + 30 min moisture heating treated + 5 g/kg xylose) and TSBM 10 g/kg (SBM + 30 min moisture heating treated + 10 g/kg xylose) treatments, respectively. Treatment of SBM with 5 and 10 g/kg of DM caused a reduction in the dry matter and effective crude protein degradability compared with NTSBM. Electrophoretic patterns of untreated and treated SBM protein residues revealed that -conglycinin , and subunit were degraded completely within 4h of incubation in the rumen, whereas the acidic and basic subunits of glycinin were degraded after 48h incubation in treated SBM with Xylose. It is concluded that SBM proteins can be effectively protected from degradation in the rumen by xylose and moisture heating treatment.
