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Biological activities of Typha capensis (Typhaceae) from Limpopo Province (South Africa)
Abstract
Dried ground leaves and rhizomes of Typha capensis were extracted with different solvents of varying polarity (hexane, dichloromethane, acetone, methanol) to determine the best extractant for subsequent
isolation and characterization of antibacterial compounds. Some extracts were active against Escherichia coli and Enterococcus faecalis, with at least one of them exhibiting minimum inhibitory
concentration values of 0.04 mg/ml. Methanol was the best extractant with an average minimum inhibitory concentration (MIC) value of 0.75 mg/ml (rhizome) and 0.21 mg/ml (leaves) for the four pathogens. E. coli and E. faecalis were the most sensitive with the average MIC values of 0.53 and 0.42 mg/ml, respectively. Staphylococcus aureus was the most resistant pathogen. The MIC values for the positive control (ampicillin) were ranging from 0.08 to 0.16 mg/ml. Average total activity, a measure of potency, was highest for methanol (4498 ml/g) leaves and (1838 ml/g) rhizomes extract followed by acetone (1795 ml/g) leaves and (1075 ml/g) rhizome extracts. The most active compounds were at the Rf value of 0.47 against all pathogens. In some extracts the antibacterial activity was high enough to consider extracts for isolation and characterisation of antibacterial compounds.
isolation and characterization of antibacterial compounds. Some extracts were active against Escherichia coli and Enterococcus faecalis, with at least one of them exhibiting minimum inhibitory
concentration values of 0.04 mg/ml. Methanol was the best extractant with an average minimum inhibitory concentration (MIC) value of 0.75 mg/ml (rhizome) and 0.21 mg/ml (leaves) for the four pathogens. E. coli and E. faecalis were the most sensitive with the average MIC values of 0.53 and 0.42 mg/ml, respectively. Staphylococcus aureus was the most resistant pathogen. The MIC values for the positive control (ampicillin) were ranging from 0.08 to 0.16 mg/ml. Average total activity, a measure of potency, was highest for methanol (4498 ml/g) leaves and (1838 ml/g) rhizomes extract followed by acetone (1795 ml/g) leaves and (1075 ml/g) rhizome extracts. The most active compounds were at the Rf value of 0.47 against all pathogens. In some extracts the antibacterial activity was high enough to consider extracts for isolation and characterisation of antibacterial compounds.
