In vitro propagation of Amsonia orientalis Decne (Apocynaceae)

Abstract

In vitro plant regeneration was achieved from shoot explants of Amsonia orientalis Decne. The seeds were germinated aseptically in Petri dishes containing growth-regulator-free MS medium. The shoot explants of 30-day-old grown seedlings were cultured on MS medium supplemented with 1 mg/l indole- 3- butryric acid (IBA) plus 0.5 mg/l kinetin (Kin) for direct regeneration and subcultured at three week intervals. While induction of adventitious buds from shoot explants was achieved approximately 33%
within four weeks, a few roots (around 15%) were induced on the same medium within eight weeks. In addition, shoot explants were transferred to MS medium containing 2 mg/l benzyl aminopurine (BAP) plus 0.2 mg/l naphtalene acetic acid (NAA) for indirect regeneration and after three weeks, proliferated actively growing calli was occured.