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Cissus Multistriata antioxidant radical scavenger polyphenols cytotoxicity Arthemia salina membrance stabilization.
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Comparative antioxidant capacity, membrane stabilization, polyphenol composition and cytotoxicity of the leaf and stem of Cissus multistriata
Abstract
In this present study, a comparative evaluation of the antioxidant capacities, phenol and polyphenol composition, membrane stabilization, and cytotoxicity to brine shrimps (Arthemia salina) of the leaf and
stem extracts of Cissus multistriata were carried out. 2,2- Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical
scavenging effect was observed in the stem extract with IC50 of 29.25 ìg/ml. The potency of radical scavenging effect of the stem extract was close to the synthetic antioxidant quercetin with IC50 of 21.05
ìg/ml. The antioxidant activities of each extract increased with increasing concentration of extracts. The phenol and polyphenol content of the stem varied between 0.564 ± 0.000 to 6.200 ± 0.557% and that of the leaf content is lower (0.523 ± 0.000 to 3.400 ± 0.355%). The higher amount of phenolic compound in the stem could be contributory to the greater radical scavenging effects observed. The methanolic extracts exhibited minimum and maximum percentage membrane stability of 51.364 ± 11.241 and 56.098
± 14.654% on human erythrocyte. The highest membrane stabilizing activity was observed in the stem extract with percentage stability of 56.098 ± 14.654%. The activity of the extracts was comparable to the
standard anti-inflammatory drug (Indomethacin) used. The extracts are less toxic to the cell (Arthenia salina) with their LC/EC50 higher than that of standard potassium dichromate used. The results obtained
in this present study indicate that C. multistriata can be a potential source of natural antioxidant and is relatively safe.
stem extracts of Cissus multistriata were carried out. 2,2- Diphenyl-1-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical
scavenging effect was observed in the stem extract with IC50 of 29.25 ìg/ml. The potency of radical scavenging effect of the stem extract was close to the synthetic antioxidant quercetin with IC50 of 21.05
ìg/ml. The antioxidant activities of each extract increased with increasing concentration of extracts. The phenol and polyphenol content of the stem varied between 0.564 ± 0.000 to 6.200 ± 0.557% and that of the leaf content is lower (0.523 ± 0.000 to 3.400 ± 0.355%). The higher amount of phenolic compound in the stem could be contributory to the greater radical scavenging effects observed. The methanolic extracts exhibited minimum and maximum percentage membrane stability of 51.364 ± 11.241 and 56.098
± 14.654% on human erythrocyte. The highest membrane stabilizing activity was observed in the stem extract with percentage stability of 56.098 ± 14.654%. The activity of the extracts was comparable to the
standard anti-inflammatory drug (Indomethacin) used. The extracts are less toxic to the cell (Arthenia salina) with their LC/EC50 higher than that of standard potassium dichromate used. The results obtained
in this present study indicate that C. multistriata can be a potential source of natural antioxidant and is relatively safe.
