Screening and characterization a RAPD marker of tobacco brown-spot resistant gene

Abstract

Bulk Segregant Analysis (BSA) and Randomly Amplified Polymorphic DNA (RAPD) methods were used to analyze F2 individuals of 82-3041 × Yunyan 84 to screen and characterize the molecular marker linked to brown-spot resistant gene. A total of 800 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Primer S361, producing one RAPD marker S361650, was tightly linked to the brown-spot resistant gene. Linkage analysis was carried out using marker S361650 on 1042 individuals of F2
progenies from the crossing between 82-3041 × Yunyan 84. The results demonstrated that the genetic distances between S361650 and brown-spot resistant gene was 2.98 cM.