Biodegradation of 2,4-dichlorophenol using Mycoplana dimorpha extracts and evaluation of kinetic parameters

Abstract

Twenty seven combinations of process variables were developed and used to produce crude extracts of Mycoplana dimorpha. Crude extracts containing 2,4-dichlorophenol degrading enzymes, were immobilized on sodium alginate beads and degradation studies was conducted in a packed bed column. The rate of degradation of 2,4-dichlorophenol by immobilized crude extracts of was measured at different time intervals and it was found that 82 to 86% of 2,4-dichlorophenol can be decomposed with different initial concentrations in 30 min. The Km and Vmax values were determined.