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Induction of callus and extraction of alkaloid from Yi Mu Cao (Leonurus heterophylus Sw.) culture
Abstract
High efficient callus was induced from cotyledon and hypocotyls of Leonurus heterophyllus Sw. on Murashige and Skoog (MS) medium containing 2 mg/l 6-benzylaminopurine (6-BA) and 0.5 mg/l 2,4-
dichlorophenxyacetic acid (2,4-D). The induction rate reached up to 94.2% in cotyledon explants and 99.2% in hypocotyls. They formed yellowish loose callus when they were subcultured on MS medium
with 2 mg/l 6-BA and 0.5 mg/l naphtaleneacetic acid (NAA). Suspension culture system was established as the callus transferred to MS liquid medium supplemented with 2 mg/l 6-BA and 0.5 mg/l NAA, and the
maximum biomass is obtained on the 16th day. The content of alkaloid extracted from suspension culture callus was only 0.081%, but the addition of 0.03 mg/l L-proline (L-Pro) could increase the content up to 2.1%.
dichlorophenxyacetic acid (2,4-D). The induction rate reached up to 94.2% in cotyledon explants and 99.2% in hypocotyls. They formed yellowish loose callus when they were subcultured on MS medium
with 2 mg/l 6-BA and 0.5 mg/l naphtaleneacetic acid (NAA). Suspension culture system was established as the callus transferred to MS liquid medium supplemented with 2 mg/l 6-BA and 0.5 mg/l NAA, and the
maximum biomass is obtained on the 16th day. The content of alkaloid extracted from suspension culture callus was only 0.081%, but the addition of 0.03 mg/l L-proline (L-Pro) could increase the content up to 2.1%.
