Production of 946-mannanase by Bacillus amylolequifaciens 10A1 cultured on potato peels

Abstract

Different Bacillus species were screened for the production of 1,4-- mannanase on solid media. Bacillus amyloliquefaciens was selected as the most potent in producing enzyme of high activity. Utilization of
various agro-industrial residues on mannanase production was evaluated. Potato peels at a concentration of 14 g/l was found to be the most effective carbon source. Maximum enzyme activity (61.5 U/mg protien) was obtained with ammonium nitrate as a nitrogen source. The optimum incubation temperature and pH for enzyme production were 35oC and 7, respectively. The influence of inoculum size was so remarkable
that, at optimum, a crude filtrate with an enzyme activity of 105.7 U/mg protien was obtained and appeared among the highest levels reported for mannanase titre. Addition of simple carbon source to media containing potato peels cause catabolic repression of mannanase synthesis.