Analysis of in vivo binding of yeast heat shock factor to promoter DNA

Abstract

The in vivo binding of yeast heat shock factor (HSF) to promoter domains of stress-inducible genes was examined using chromatin immunoprecipitation (ChIP) combined with PCR. The promoter-specfic PCR amplification of DNA immunoprecipitated with antibodies against HSF showed that yeast HSF was bound constitutively to the promoters of GTT2, YLL058W, COQ6, GND2 and YGR066C genes, induciblely to the MRS2 gene. HSF-binding consensus sites prediction indicated the promoter sequences of the stress-inducible genes contain heat shock elements (HSEs). These results suggest the genes are potential HSF-regulated targets.