Biological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum Sant. et Fernand.

Abstract

The micro-cloned plantlets of Chlorophytum borivilianum registered more than 95% establishment in soil following treatment with various bio-inoculants namely; Glomus aggregatum, Trichoderma harazianum and Piriformospora indica whereas Azospirullum sp. (CIM-azo) and Actinomycetes sp. (CIM-actin) showed only up to 85% plantlet establishment. The un-rooted shoots were also treated with
these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with
mycorrhiza Glomus aggregatum (VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.