Hormonal regulation for callogenesis and organgenesis of Artemisia absinthium L

Abstract

Callus cultures were induced from leaf and stem explants of Artemisia absinthium, at different auxin and cytokinin concentrations. Moderate concentrations of growth regulators either in combination or in
single in MS medium produced friable, light green and non-embryogenic callus from both explants. These totipotent cells gave rise to shoots when transferred to same or different growth regulator containing medium as second subculture. Complete rooting was achieved on full and half strength basal MS medium supplemented with different auxin concentrations. Synergetic effect of plant growth regulator plays an important role in callus induction and cell differentiation.