Optimization of FTA technology for large scale plant DNA isolation for use in marker assisted selection

Abstract

Conventional methods for DNA acquisition and storage require expensive reagents and equipments. Experimental fields located in remote areas and large sample size presents greater challenge to developing country institutions constrained financially. FTATM technology uses a single format utilizing basic tools found in laboratory. In this study, FTATM cards were used to collect over 3000 samples from a field located 130 km and used to screen progeny plants using PCR-marker-assisted selection. PCR product yields and quality are sufficient for reliable scoring, distinguishing heterozygous from
homozygous plants using ABI 3730 sequencer. Results indicate that this method is faster, easier and in-expensive.