Response surface methodological approach to optimize the nutritional parameters for enhanced production of
-amylase in solid state fermentation by Thermomyces lanuginosus

Abstract

Response surface methodology was used to study the cumulative effect of the nutritional parameters and to enhance the production of extracellular -amylase in solid-state ermentation by Thermomyces lanuginosus ATCC 58157. These nutritional parameters considered include carbon source (soluble starch), nitrogen source (peptone) and a concentrated mineral medium. For obtaining the mutual interaction between the variables and optimizing these variables, a 2³ factorial central composite design using response surface methodology was employed. The optimal calculated values of tested variables for maximal production of -amylase were: soluble starch, 71.10 g/Kg peptone, 91.97 g/Kg and mineral salts solution, 175.05 ml/Kg with a predicted -amylas activity of 5.085 ´ 10⁵ U/Kg of wheat bran. These predicted optimal parameters were tested in the laboratory and the final -amylase activity obtained, 4.94 ´ 10⁵ U/Kg of wheat bran, was very close to the predicted value.

African Journal of Biotechnology Vol. 4 (7), pp. 708-716, 2005