Isolation and characterization of micro satellites in Bambusa arundinacea and cross species amplification in other bamboos

Abstract

Isolation and characterization of microsatellites was analyzed in Bambusa arundinacea and ross species amplification in other bamboos. Six microsatellites, three polymorphic and three onomorphic, were characterized in a bamboo species, Bambusa aruninacea belonging to the family Poaceae. The numbers of alleles per locus ranges form 2 to 6. Allelic diversity ranges from 0.128 to 0.789. Polymorphic Information Content (PIC) values for two loci were > 0.3, as an indicator of polymorphic allele. Cross species amplification has been tested in other 18 bamboo species. Monomorphic simple sequence repeats (SSRs) have been found to be cross amplified in most of the tested species while polymorphic ones in only three to four species. Utilization of the SSR loci in genetic diversity study of B. arundinacea and other cross amplified bamboo species is discussed.

African Journal of Biotechnology Vol. 4 (2), pp. 151-156, 2005