Boukaré Zeba
Laboratoire de Biochimie et de Chimie appliquée/ Enzymologie. UFR-SVT/ Université de Ouagadougou/ Burkina Faso
Pere Jacques Simporé
Camille medical center/ Ouagadougou/ Burkina Faso
Odile G Nacoulma
Laboratoire de Biochimie et de Chimie appliquée/ Enzymologie. UFR-SVT/ Université de Ouagadougou/ Burkina Faso
Jean-Marie Frere
Enzymologie/ Centre d’ingenierie des protéines / Ulg/ Belgique
Abstract
Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β--lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene.
Key words: β--Lactamases, Klebsiella pneuminiae, blaSHV gene, Saint Camille medical centre, Ouagadougou.
African Journal of Biotechnology Vol.3(9) 2004: 477-480