Callus induction and growth in transgenic potato genotypes

Abstract

The aim of this study was to establish an effective protocol for callus induction from the potato genotypes (Solanum tuberosum L.) used and to investigate whether the transferred oxalate oxidase enzyme or transformation procedure has any effect on callus induction of transgenic lines of cultivar Desiree and Maris Bard. The results showed that the effects of genotype and medium on measured characters were highly significant. In order to compare overall performance of transgenic lines of each cultivar with their parental cultivars (non-transgenic), orthogonal comparisons were used and indicated the significant differences between transgenic and non-transgenic genotypes. The significant differences between the cultivars and their transgenic lines expressing oxalate oxidase enzyme indicated that the transformation procedure had a significant effect on their callus induction and growth.

Key words: Callus, Oxalate oxidase, solanum tuberosum, transformation.

African Journal of Biotechnology Vol.3(8) 2004: 375-378