CO Nweke
Department of Microbiology, University of Port Harcourt, P. M. B. 5323, Port Harcourt, Nigeria
GC Okpokwasili
Department of Microbiology, University of Port Harcourt, P. M. B. 5323, Port Harcourt, Nigeria
Abstract
Staphylococcus sp. isolated from oil-contaminated soil was grown in 1% drilling fluid base oil, HDF- 2000, as a sole source of carbon and energy. The organism has strong affinity for the substrate, growing at the rate of 0.16 h-1. It uses adherence and emulsification as mechanisms for oil uptake. In a nutrient-rich marine broth, base oil (up to 2.0% v/v) and glucose (up to 1.6% w/v) have no significant effect on the growth rates. This showed that the Staphylococcus sp. is a strong primary utilizer of the base oil and has potential for application in bioremediation processes involving oil-based drilling fluids.
Key words: Drilling fluid base oil, Staphylococcus sp., biodegradation
African Journal of Biotechnology Vol.2(9) 2003: 293-295