Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia

Abstract

This study was aimed to analyse the genetic polymorphism of Growth Hormone (GH) polymorphism of Simmental cattle using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) method and its relation to average daily gain. The research was conducted in the Padang Mangatas Breeding Centre, Limapuluh Kota district, West Sumatera Province and Biotechnology Laboratory of Faculty of Animal Husbandry, Andalas University. The research used 100 Simmental calves. DNA were isolation from blood sample using DNA purification Kit from Pomega. The PCR procedure was used to amplify 591-bp of bGH exon 1 (GH1) and 694-bp exon 2 (GH2). The PCR product were digested by restriction enzymes MspI and AluI. Digestion of 591-bp GH gen PCR product with enzyme restriction MspI reveal allele A(+) and B(-) with frequency 0.875 and 0.125 respectively and digestion with restriction enzyme AluI revealed allele C(+) and D(-) with frequency 0.95 and 0.05 respectively and. Digestion of 694-bp PCR product by MspI represent allele P(+) and Q(-) with frequency 0.88 and 0.12 respectively and digestion with AluI enzyme represent allele R(+) and S(-) with frequency 0.94 and 0.06 respectivly. The observed heterozygosity, effective allele numbers and polymorphism information content of GH1/MspI, GH2/MspI, GH1/AluI, and GH2-AluI were 0.11/0.1948, 0.04/0.1889, 0.00/0.0927, and 0.00/0,1096 respectively. Using GLM, there was no relation between these polymorphic and avarage daily gain of calve.

Key words: Simmental cattle, growth hormone (GH) gene, polymorphism, avarage daily gain growth.