Development of bio-hybrid material based on Salmonella Typhimurium and layered double hydroxides

Abstract

The immobilization of a whole microbial cell is an important process used in nanotechnology of biosensors and other related fields, especially the development of bio-hybrid materials based on live organisms and inorganic compounds. Here, we described an essay to develop a bio-hybrid material based on Salmonella Typhimurium cells and layered double hydroxides (LDH). The synthetic clays have a good capacity to be a host matrix for immobilization of live entity like bacteria. The incorporation of LDH in the nutritive broth shows the capacity of bacteria to grow under the inorganic conditions. The immobilization of bacteria onto the LDH Layer deposited on gold wafers was successfully done and the verification of the final material consistence was given by Fourier transform infrared spectroscopy (FTIR) analysis that shows the possibility of various covalent links that can be established between the polar functional group of the cell and the interlayer level in the LDH. The roughness of the surface was given by scanning electron microscope (SEM) imaging and shows the homogeneity of cell distribution on the LDH layer.

Keywords: Layer double hydroxide, Salmonella Typhimurium, Fourier transform infrared spectroscopyattenuated total reflectance (FTIR-ATR), X-ray diffraction (XRD), energy dispersive X-ray (EDX); scanning electron microscope (SEM)