One step method of plantlet regeneration in Trichosanthes dioica Roxb.: An approach towards cost effective and shorter protocol

Abstract

In order to reduce the cost and time of in vitro raised plants of Trichosanthes dioica Roxb., a minimal medium has been formulated by substituting costly growth regulators from the medium with a cost effective constituent, the coconut milk. A semisolid Murashige and Skoogs’s medium supplemented exclusively with 15% coconut milk showed the highest percentage of plantlet regeneration (99%) in the explants. When nodal, shoot-tip and immature leaf explants were cultured on this medium, rhizogenesis was observed in about 5 to 6 days of inoculation, followed by shoot formation in about 8 to 10 days. The fully developed plantlets, 10 to 12 cm in length with professed roots were obtained in about 20 days of inoculation in a single step without adding/changing growth regulators. After transplantation in the potted soil, these plantlets showed similar growth patterns as compared to the plants obtained from a conventional three-four step method of tissue culture experiments.

Keywords: Coconut milk, tissue culture, Trichosanthes dioica Roxb