In vitro anti-inflammatory and phytochemical properties of crude ethyl acetate extract of Baliospermum montanum Leaf (Muell – Arg)

Abstract

Baliospermum montanum (Muell – Arg) which belong to Euphorbiaceae family is a well known perennial herb in Indian medicine used to treat various disorders like asthma, bronchitis, purgative, anthelmintic, diuretic, diaphoretic, rubefacient and tonic. The anti-inflammatory activity of four different solvent extracts of B. montanum leaf was investigated in Phytohaemagglutinin (PHA) induce peripheral blood mononuclear cells (PBMC) cells using MTT assay. Among the samples, ethyl acetate extract revealed good anti-inflammatory response comparatively with other extracts such as hexane, acetone, methanol and the preliminary screening of phytochemical test was investigated. The results of phytochemical screening revealed the presence of flavonoids, tannin, steroids, glycosides, amino acid and carbohydrates. Our study demonstrates that the ethyl acetate extracts of B. montanum leaves contains effective  anti-inflammatory agents, which could ultimately be used as functional material and traditional remedy against inflammation.

Keywords: Baliospermum montanum, anti-inflammatory, MTT assay, phytochemical

African Journal of Biotechnology Vol. 12(39), pp. 5743-5748