Determination of genetic polymorphism among indigenous and exotic maize inbreds using microsatellite markers

Abstract

Maize (Zea mays L.) is an important cereal crop of different countries of world. Undoubtedly, the concerted efforts of plant breeders and their breeding strategies have helped to increase the production and productivity to the tune of two to three folds in many crops including maize. Hybrid cultivars have played a vital role in increasing the acreage and productivity of maize. The success in identifying heterotic hybrid in maize hybrid breeding depends on the availability of genetically diverse maize inbred lines developed from different heterotic gene pool. Hence, generation of information on variability of inbreds at genotypic level become necessary. Molecular markers have proven to be a valuable tool for assessing the genetic diversity in many crop species. Simple sequence repeats (SSR) are currently considered as the molecular markers of choice and are rapidly being adapted by plant researchers for precise estimation of diversity. SSR based molecular diversity analysis of 27 maize inbred lines had produced 23 polymorphic alleles from 10 markers with an average of 2.3 alleles per locus and mean polymorphic information content (PIC) of 0.45. The dendrogram generated with hierarchical unweighted pair group method with arithmetic mean (UPGMA) cluster analysis revealed five major clusters at 0.62 similarity coefficient. The information on diversity of inbred lines generated in this study would be much useful in developing heterotic hybrids.

Keywords: Maize, inbreds, marker diversity, simple sequence repeats (SSR)

African Journal of Biotechnology Vol. 12(39), pp. 5723-5728