DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

Abstract

In the present study, genetic variability in 10 commercial chilli pepper varieties viz. Gemini, G-334, Agnirekha, Pusa jwala, Mangala, Black diamond, Sindura, Pusa jyothi, Badiga-2 and Teja (branch), cultivated in the local area of Andhra Pradesh State, India, using random amplified polymorphic DNA (RAPD) markers was examined. Out of thee RAPD markers used for screening 10 chilli pepper genotypes, DNA amplification was observed only with OPAB02; this primer produced monomorphic band in Gemini,G-334, Black diamond and Sindura and polymorphic bands in Agnirekha, Pusa jwala, Mangala, Pusa jyothi, Badiga-2 and Teja (Branch). Genetic variabilty in terms of DNA pattern produced in the above varieties could be used as a marker to distinguish between them. Dendrogram generated by OPAB02 primer showed that the 10 chilli pepper varieties could be grouped into four clusters. Average genetic similarity index revealed 100% similarity between varieties of first cluster, 50% similarity between varieties of second and fourth clusters and 32% genetic similarity between varieties of third cluster. UPGMA cluster analysis will be useful in chilli pepper breeding programmes and germplasm conservation. Since the commercial value of chilli pepper is based on pungency level, future studies are aimed at molecular marker based pungency phenotyping.

Keywords: Capsicum annuum, Randomly Amplified Polymorphic DNA, Dendrogram, Polymerase Chain Reaction.

African Journal of Biotechnology Vol. 12(30), pp. 4730-4735