Integrated management of Fusarium wilt of chickpea (Cicer arietinum L.) caused by Fusarium oxysporum f. sp. ciceris with microbial antagonist, botanical extract sp. ciceris with microbial antagonist, botanical extract

Abstract

The present study was carried out to assess the efficacy of an integrated management strategy for Fusarium wilt of chickpea that combined the use of microbial antagonist, botanical extract and fungicide. Before setting the experiment in field micro plots, a series of in vitro and in vivo experiments were conducted to select a virulent isolate of F. oxysporum f. sp. ciceris, an effective antagonistic isolate of Trichoderma harzianum, a fungitoxic botanical extract and an appropriate fungicide. The isolate FS1 of F. oxysporum f. sp. ciceris appeared to be most virulent to chickpea cultivar BU-Chola-1 and selected as test pathogen. Among the 20 isolates screened, T. harzianum isolate T-75 showed the highest (75.89%) inhibition of the radial growth of F. oxysporum f. sp. ciceris in dual culture assay on PDA. Absolute inhibition (100.00%) of colony growth of F. oxysporum f. sp. ciceris was observed where fungicide Provax-200 at 100 ppm was used. Azadirachta indica leaf extract gave maximum inhibition (55.19%) of radial growth of F. oxysporum f. sp. ciceris at all concentrations. The integration of soil treatment with T. harzianum isolate T-75 and Az. indica leaf extract and seed treatment with Provax-200 appeared to be significantly superior in reducing Fusarium wilt and in improving seed yield of chickpea compared to any single or dual application of them in the field. The results of this study exhibit the importance of integrating selective microbial antagonist, botanical extract and fungicide to achieve appropriate management of Fusarium wilt and increase of seed yield in chickpea in Bangladesh.

Keywords: Integrated management, Fusarium wilt, Fusarium oxysporum f. sp. ciceris, chickpea (Cicer arietinum L.), antagonists, botanicals, fungicides.

African Journal of Biotechnology Vol. 12(29), pp. 4699-4706