Photosynthetic behaviour of Arabidopsis thaliana (Pa-1 accession) under salt stress

Abstract

The growth reduction observed in many plants caused by salinity is often associated with a decrease in their photosynthetic capacity. This effect could be associated with the partial stomatal closure and/or the non-stomatal limitation which involves the decrease in ribulose-1,5-bisphosphate carboxylase oxygenase (RUBISCO) activity. The objective of this study was to explore the mechanisms of inhibited photosynthesis in Arabidopsis thaliana (Pa-1 accession) under salt stress. Pa-1 seeds grown on a solid substrate for 25 days on standard medium were challenged with 50 mM NaCl for 15 days. Harvests were carried out every five days by separating the rosette leaves and roots. Salt stress reduced growth by limiting the number of the rosette leaves and not their biomass. Accumulation of Na+ and Cl- increased during the treatment period, whereas K+ and Ca2+ accumulation were reduced in salt treatment. RUBISCO and phosphoenolpyruvate carboxylase (PEPC) activities were increased with the age of the leaves to a maximum after 10 days of treatment then later decreased. We concluded that the sensitivity of Pa-1 to salinity may be due to a reduction in number of leaves, in the photosynthetic assimilation with stomatal closure and damage of the RUBISCO and PEPC activities.

Keywords: Arabidopsis thaliana, photosynthetic parameters, salinity, ribulose-1,5-bisphosphate carboxylase oxygenase (RUBISCO), phosphoenolpyruvate carboxylase (PEPC).

African Journal of Biotechnology Vol. 12(29), pp. 4594-4602