Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment

Abstract

Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top^® and CoMo Raiz^® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitro. The experimental design was completely randomized in a 4 × 3 + 1 factorial arrangement (four concentrations: 1, 2, 3, and 4 mL L^-1 of the commercial products Standak Top^®, CoMo Raiz^®, a mixture of both, and a control treatment) with 20 replicates. The following characteristics were evaluated: Contamination level, shoot length, and the number of leaves. No contamination was observed for the different doses of Standak Top^®. Additions of 1 to 3 mL L^-1 of Standak Top^® to the culture media yielded the greatest shoot length, and the additions of 2 to 3 mL L^-1 yielded the greatest number of leaves. Mixing Standak Top^® and CoMo Raiz^® did not significantly enhance the measured characteristics.

Keywords: Standak Top^®, CoMo Raiz^®, tissue culture, asepsis, culture media

African Journal of Biotechnology Vol. 12(25), pp. 3963-3967