Could grape seed extract modulate nephritic damage induced by methomex in male rats?

Abstract

Methomex (Metho) is classified as a carbamate insecticide. The present study was designed to examine the influence of grape seed oil (GSO) on the histopathological changes in methomex-induced kidney damage in male rats. Rats were divided into 6 groups, the first of which was considered as the control. The 2nd group was treated with 4 ml/kg GSO. Rats from 3^rd and 4^th groups were treated with Metho at dose level of 2.4 and 4.8 mg/kg, respectively. Rats from 5^th and 6^th groups pre-administered with GSO were treated with 2.4 and 4.8 mg/kg Metho, respectively. Metho administration caused destruction of the normal pattern of the renal tissue. These damages were encountered by the presence of some glomeruli appeared atrophy with distension of Bowman’s space and degeneration of their parietal epithelial cells. The lumina of distal and proximal convoluted tubules contain hyaline casts of dead cells. The renal medulla showed dilated collecting tubules stuffed with red blood cells (RBCs). Preadministration of GSO to Metho-induced rats revealed apparent normal renal parenchyma. The proximal convoluted tubules and collecting tubules appeared near to normal with their narrow lumen. Preadministration with GSO exhibited that it had a protective effect against methomex-induced toxic effects in the kidney. The present study advocated using GSO in the daily diets.

Key words: Grape seed oil, methomex, kidney, histopathology.