RAPD-PCR analysis of some species of Euphorbia grown in University of Baghdad Campus in Jadiriyah

Abstract

This study attempts to identify species of Euphorbia (Euphorbia peplus, Euphorbia helioscopia, Euphorbia granulata and Euphorbia hirta) grown in University of Baghdad Campus in Jadiriyah and determine the genetic polymorphism among them by using DNA markers generated by polymerase chain reaction (PCR). Total genomic DNA of species studied was extracted from dry seeds by using commercial kit. Molecular analysis was performed by using nine random markers in random amplified polymorphic DNA (RAPD-PCR) technique. RAPD-PCR analyses based on three primers A13, C05 and D20 gave results in term of amplification and polymorphisim for the four species studied. The genetic polymorphisms value of each primer was determined and ranged between 47 to 84%; primer A13 produced the highest percent of genetic polymorphism compared with primer C05. RAPD-PCR technique confirmed the isolation of the four species of Euphorbia obviously.

Key words: Euphorbia spp., random markers in random amplified polymorphic DNA (RAPD-PCR), monomorphic, polymorphic, random primers.