Development and characterization of nine polymorphic microsatellite markers in the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae)

Abstract

In this study, nine microsatellite loci were isolated and characterized from the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae). The loci were validated and characterized using 20 samples collected from five Korean localities. These results indicate that some loci were highly variable in terms of number of alleles (2 to 13), heterozygosity (0.10 to 0.40), and polymorphic information content (0.31 to 0.85). These microsatellite markers will be very valuable for population genetic studies of C. septempunctata.

Key words: Seven-spotted lady beetle, Coccinella septempunctata, microsatellite Deoxyribonucleic acid (DNA).