Genetic diversity study of indigenous cattle (Gir and Kankrej) population of Rajasthan using microsatellite markers

Abstract

The genetic diversity study of native Gir and Kankrej (Bos indicus) cattle populations were evaluated using nine microsatellite markers (ETH-225, CSRM-60, HEL-9, INRA-005, ETH-10, HAUT-24, BM1818, ILSTS-002 and ILSTS-006) suggested by FAO (ISAG). A total of 60 cattle were sampled from different places of local Rajasthan region. For each, 30 individuals were sampled. The mean number of observed and effective alleles in Kankrej were high (5.222 and 3.714) comparatively and the average expected heterozygosity values (0.5403) indicated high diversity in the Kankrej population than Gir (0.4520). High polymorphism information content (PIC) values observed for most of the markers with an average of 0.5116 are indicative of high polymorphism of these markers in Kankrej breed than in Gir (0.4202), which showed high informativeness of all the microsatellite markers in Kankrej breed. Three microsatellites markers (HAUT24, BM1818 AND ILSTS006) did not show amplification in both breeds. INRA005 was the only markers amplified in Kankrej. The allele diversity (mean observed number of alleles was 6.11; mean effective number of alleles was 5.187) and gene diversity (0.2771) values implied a substantial amount of genetic variability in both populations. Reasonably high PIC values observed for most of the markers, with an average PIC value of 0.5116 across all the loci implied that this set of microsatellite are very informative for evaluation of genetic diversity in both the breeds. This informativeness of microsatellite markers showed it can be used for various applications like, conservation, disease diagnosis and polymorphism in different populations.

Key words: Genetic diversity, polymorphism information content (PIC), heterozygosity, polymorphism, marker.