Assessment of genetic diversity of nine hybrid combinations of canola using SDS-PAGE, RAPD and ISSR markers

Abstract

Genetic variations of nine hybrid combinations of canola were determined using sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE), random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. The protein profile did not show any polymorphism. The RAPD and ISSR primers recorded a high degree of polymorphism among generations of the three canola hybrids. A  maximum of 48 DNA bands were detected as polymerase chain reaction (PCR) products for the six RAPD primers from which 42 were polymorphic with mean polymorphism percentage of 82.6%. The four ISSR  primers produced 25 bands, of which 23 were polymorphic. The percentage of polymorphism using ISSR  primers ranged from 66.6 to 100.0% with an average of 85%. Using cluster analysis, the 9 canola generations  were categorized into single generation, F3 (P1 × P3) formed a separate operational taxonomic units (OTU) in cluster and one main cluster that was further divided into three sub clusters. The results show high variation within and among hybrids.

Key words: Canola, generations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), genetic diversity.